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Use of gradient elution for rapid selection of isocratic conditions in reversed phase HPLC
Summary
Type:
HPLC
Application #:
107620
Catalog#:
720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection:
UV, 254nm
Conditions:
eluent: methanol - water, acetonitrile - water and tetrahydrofuran - water as indicated, 1mM perfluorooctanoic acid added in B, C and D
flow rate 1.5ml/min
Authors:
Schoenmakers, P.J. et al.
Source:
J. Chromatogr. 205 (1981) 13–30
Details
Legend: Fig. 14. Isocratic elution of PTH amino acids, 1. glycine, 2. alanine, 3. arginine, 4. glutamic acid, 5. methionine, 6. phenylalanine, 7. isoleucine, 8. leucine.
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